13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 3

TITLE: Effect of Low Laser Therapy on the viability of fibroblast and osteoblast submitted to sodium alendronate in culture.

AUTHORS: Brozoski MA, Marques MM, Ferreira SF, Azambuja-Júnior N, Traina AA, Naclério-Homem MG.

Universiyt of São Paulo

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S151.

* doi:10.4317/medoral.17643650

http://dx.doi.org/10.4317/medoral.17643650

Abstract

The management of bisphosphonates induced osteonecrosis of jaw (BONJ) remains a challenge. Literature describes different forms of treatment and the majority of the studies agree in minimal invasive procedures. It is known that laser phototherapy is able to increase cell metabolism and therefore could be an alternative for BONJ handling. This study aimed to evaluate the effect of LPT on the viability of osteoblasts (OB) and fibroblasts (FB) treated with sodium alendronate, a bisphosphonate used for osteoporosis treatment. Cells stayed in contact with DMEM containing sodium alendronate for 24 hours. After this period, cells where submitted to three sessions of LPT with a continuous diode laser (InGaAlP, 660nm, beam spot area of 0.028cm²) in punctual and contact mode using the following parameters: 40mW, 6J/cm² or 18J/cm², 4 or 12s and 0.16 or 0,48J per point, respectively. Cell viability and proliferation were determined by MTT assay in 24h, 48hs, 72h after the first irradiation. It was possible to conclude that alendronate is cytotoxic to OB and FB in culture. Significant cell growth was observed in both irradiated OB and FB cultures, in particular when for the energy density of 6J/cm² was used. Based on the experimental conditions and laser parameters tested in this study it is possible to conclude that LPT is a promising treatment for BONJ in terms of increasing cell viability of FB and OB affected by bisphosphonates.