13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 8

TITLE: Evaluation of bone healing on tibial fractures in rabittis: laser fluorescence analysis.

AUTHORS: Aciole JMS, Aciole GTS, Soares LGP, Rodrigues LFB, Santos JN, Pinheiro ALB.

Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S156.

* doi:10.4317/medoral.17643656

http://dx.doi.org/10.4317/medoral.17643656

Abstract

The aim of this study was to evaluate, through the analysis of fluorescence laser, the repair of complete tibial fracture in rabbits fixed with internal rigid fixation (IRF), treated or not with infrared laser light (λ 780 nm, 50mW, CW) associated or not to the use of hydroxya-patite and guided bone regeneration (GBR). Before the surgical procedure was performed the measurement with the DIAGNOdent device to see the degree of bone demineralization. Surgical fractures were created, under general anesthesia (Ketamine/Xilazine), on the dorsum of 15 Oryctolagus rabbits that were divided into 5 groups. On groups II, III, IV and V the fracture was fixed with wire miniplates (IRF). Animals of groups III and V were grafted with hydroxyapatite and GBR technique used. Animals of groups IV and V were irradiated at every other day during two weeks (16J/cm², 4 x 4J/cm2). After confirmation of the death of animals (30 days postoperatively), we proceeded to measure the fluorescence lase r bone through the use of DIAGNOdent® 2095 (Kavo, Biberach, Germany, λ655nm, 1mW) according to manufacturers recommendations. The readings were taken at four equidistant points on the operated area and the results recorded in standard form. The laser fluorescence analysis showed a lower degree of demineralization when laser was associated to the HATCP. It is concluded that IR laser light was able to accelerate fracture healing and the association with HATCP and GBR resulted on increased deposition of CHA.