13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 9

TITLE: Assessment of bone healing on tibial fractures in rabittis using internal rigid fixation, laser and hydroxyapatite: raman spectroscopy analysis.

AUTHORS: Aciole JMS, Aciole GTS, Soares LGP, Rodrigues LFB, Silveira Jr L, Pinheiro ALB.

Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S157.

* doi:10.4317/medoral.17643657

http://dx.doi.org/10.4317/medoral.17643657

Abstract

The aim of this study was to evaluate, through the analysis of Raman spectroscopy, the repair of complete tibial fracture in rabbits fixed with internal rigid fixation (IRF), treated or not with infrared laser light (λ 780nm, 50mW, CW) associated or not to the use of hydroxyapatite and guided bone regeneration (GBR). Surgical fractures were created, under general anesthesia (Ketamine/Xilazine), on the dorsum of 15 Oryctolagus rabbits that were divided into 5 groups. On groups II, III, IV and V the fracture was fixed with wire miniplates (IRF). Animals of groups III and V were grafted with hydroxyapatite and GBR technique used. Animals of groups IV and V were irradiated at every other day during two weeks (16J/cm², 4 x 4J/cm²). Observation time was that of 30 days. After animal death (overdose of general anesthetics) the specimens were kept in liquid nitrogen and used for Raman spectroscopy. The Raman spectroscopy readings showed significant differences between groups (p<0.001) in relation on group IRF, showing a greater deposition of hydroxyapatite. It is concluded that IR laser light was able to accelerate fracture healing and the association with HATCP and GBR resulted on increased deposition of CHA.