13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 11

TITLE: Effect of photobiomodulation led (λ850nm) on bone repair in ovariectomized rats: Raman Spectroscopy.

AUTHORS: Aciole JMS, Aciole GTS, Carvalho FB, Barbosa AFS, Rodrigues LFB, Pinheiro ALB.

Center of Biophotonics, School of Dentistry, Federal University of Bahia, 62 Araujo Pinho Ave, Canela, Salvador, BA, Brazil, 40110-150

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S159.

* doi:10.4317/medoral.17643659

http://dx.doi.org/10.4317/medoral.17643659

Abstract

LED phototherapy has shown positive results in bone repair as it increases blood supply to the damaged area. We used Raman spectroscopy to assess the the effect of λ850nm LED light on surgical bone defects in the femur of ovariectomized rats. 30 Wistar rats were equally divided into 6 groups. Aside from the normal baseline group, all groups suffered osteoporosis induced by ovariectomy (OVX). Under general anesthesia, a bone defect (5mm²) was created in the femur of each animal. Animals in the osteoporotic Basal Group (IG) were sacrificed 60 days after OVX; normal basal group (GII): OVX or no preparation of defect, were killed along with the GI group, the control group (GIII): 60 days after OVX, the bone defect was created and submitted to simulated irradiation by LED, LED Group (GIV): 60 days after OVX, the bone defect was created and submitted to irradiation with LED (λ850nm, 150mW, Ø 0.5 cm², 20.4 J/cm², 68s), Group LED + GBR (GV): 60 days after OVX, the same defect was covered with a bovine bone membrane further irradiated with LED. The irradiation protocol was carried out at every other day during 15 days. The animals were sacrificed on the 15th postoperative day, specimens were removed, immersed in liquid nitrogen and sent for Raman analysis. We detected significant higher concentration of hydroxyapatite in GIV group (p <0.001) and the lowest observed in the GV. It was concluded that LED phototherapy accelerated bone repair in ovariectomized rats.