13th World Congress for Laser Dentistry
26-28, April 2012
Barcelona, Spain
Meeting Abstract
Sponsors: World Federation for Laser Dentistry
- Poster 16
TITLE: Raman Study of the Effect of LED light on
Grafted Bone Defects.
AUTHORS: Soares LGP, Aciole JMS, Aciole GTS, Barbosa
AFS, Santos JN, Pinheiro ALB.
Center of Biophotonics, School of
Dentistry, Federal University of Bahia
SOURCE: Med
Oral Patol Oral Cir Bucal.
2012 May 1;17(Supplement1):S164.
* doi:10.4317/medoral.17643664
http://dx.doi.org/10.4317/medoral.17643664
Abstract
Aim: We carried out a Raman spectral analysis
on surgical bone defects grafted or not with Hydroxyapatite (HA), treated or
not with LED. We have used several models to assess the effects of laser on
bone. Background: Benefits of the isolated or combined use them on bone healing
has been suggested. Material and Methods: 40 rats were divided into 4 groups.
On Group I the defect was filled with the clot. On Group II, the defect was
filled with the HA. On groups III the defect was filled with Clot and further
irradiated with LED and on group IV the defects was filled with the HA and
further irradiated with LED. LED (λ850 ± 10nm, 150mW, A= 0.5cm2, 68s, 20 J/cm2 per session, 140 J/cm² per treatment) was
applied at 48 h intervals during 15 days. Specimens were taken after 15 and 30
days after surgery and kept on liquid nitrogen, and underwent Raman analysis.
For this, the peak of hydroxyapatite (˜960 cm-1) was used as marker of bone
mineralization. Results: Significant difference was observed at both times
(p<0.05). When the biomaterial was used higher peaks were observed.
Association with LED further improved the intensity. Conclusion: It is
concluded that LED light improved the effect of the HA.