13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 29

TITLE: Use of Raman Spectroscopy in the validation of DIAGNOdent used in the diagnosis of caries.

AUTHORS: Carvalho FB, Barbosa AFS, Aciole JMS, Silveira Jr L, Pinheiro ALB.

Center of Biophotonics, School of Dentistry, Federal University of Bahia

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S177.

* doi:10.4317/medoral.17643677

http://dx.doi.org/10.4317/medoral.17643677

Abstract

Dental caries is one of the most prevalent diseases in oral cavity, involving progressive demineralisation and proteolytic degradation of organic matrices. The lack of a method with sensitivity and specificity led to the development of methods of diagnosis such as laser fluorescence DIAGNOdent (KaVo, Biberach, Germany). This work was to verify by Raman spectroscopy if there is a correlation between the levels of hydroxyapatite (960 cm-1), fluoridated apatite (575 cm-1) and organic matrix (1450 cm-1) and the values on DIAGNOdent at different stages of dental caries in extracted human teeth. The peak value of fluorescence in the area of caries was recorded and teeth divided into enamel caries, in dentin and healthy tooth. After DIAGNOdent reading the teeth were taken to carry out the Raman spectroscopy. Data were analyzed by Anova and Pearson correlation. The results showed significant difference between teeth with caries and without caries for all Raman peaks (p<0.01) and between groups of the DIAGNOdent (p <0.01) with higher readings for dentin caries. The Raman peaks had positive correlation (p <0.05) for both caries in dentin and enamel. Peaks of 575 and 960 cm-1 were more intense in caries in ena-mel than in dentin caries. There was significant negative correlation between the peaks 575 and 960 cm-1 and dentin caries. It can be concluded that the higher the fluorescence detected by Diagnodent lower the peak of hydroxyapatite and fluoridated apatite.