13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 37

TITLE: Antimicrobial activity of active fractions from Eleagia utilis and Isertia laevis against S. mutans and S. sobrinus.

AUTHORS: Gamboa F, Aldana J, Saavedra L, Tellez M, Perdomo M, Tellez N.

Grupo de Investigacion en Fitoquimica, Dpto de Microbiologia y Centro de Investigaciones Odontologicas. Pontificia Universidad Javeriana. Bogota

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S185.

* doi:10.4317/medoral.17643685

http://dx.doi.org/10.4317/medoral.17643685

Abstract

Introduction: dental caries is considered an infectious disease leading to the destruction of dental hard tissue. The major bacterial species involved in dental caries is Streptococcus mutans, which has led the implementation of prevention and control measures. The fundamental goal of research on medicinal plants is the search for substances or compounds with antimicrobial activity. Aim: to evaluate the antimicrobial activity of fractions obtained from two plant species, Elaeagia utilis and Isertia laevis against S. mutans and S. sobrinus. Material and methods: from the ethanolic extract of leaves of E. utilis and I. laevis were obtained fractions by two extraction methods. The evaluation of the antimicrobial activity of fractions against S. mutans and S. sobrinus was undertaken by the well diffusion method and bioautography. Results and conclusion: fractions obtained from E. utilis showed antimicrobial activity from the essential oil extracted on S. mutans at a final concent ration of 1mg/well. The dichloromethane fraction of ethanolic extract was the most active, from this was obtained a subfraction with inhibitory activity at concentrations up to 0.1mg/well. The active fractions of I. laevis showed antimicrobial activity with minimum inhibitory concentration of 2 mg/well and 1 mg/well on S. mutans and S. sobrinus. Two compounds of type triterpene saponins and steroid were isolated, which showed a minimum inhibitory concentration of 0.4 mg/well for both microorganisms.