13th World Congress for Laser Dentistry
26-28, April 2012
Barcelona, Spain
Meeting Abstract
Sponsors: World
Federation for Laser Dentistry
- Oral
Presentation 19
TITLE: The effects of Er:YAG and diode laser on Enterococcus
Faecalis: an in vitro study.
AUTHORS: Telesca
V, Palaia G, Tenore G, Berlutti F, Romeo U.
SOURCE:
Med Oral Patol Oral Cir Bucal.
2012 2012 1;17(Supplement1):S19.
* doi:10.4317/medoral.17643518
http://dx.doi.org/10.4317/medoral.17643518
Abstract
The aim of this study was to test, by an “in
vitro” experience, the antimicrobial activity of two different wavelength
lasers (Diode 808nm and Er:YAG)
Introduction : The use of lasers in endodontic
treatment has been increased in recent years, especially thanks to their
antimicrobial abilities that have been investigated in vitro.
Aim of the study: The aim of this study was
to test, by an “in vitro” experience, the antimicrobial activity of two
different wavelength lasers (Diode 808nm and Er:YAG 2940nm) against Enterococcus
faecalis.
Materials and methods
:Thirty root canals were consi-dered for the
experiment. All teeth were sterilized by autoclaving at 134° C, then they were infected artificially with colonies of E. faecalis and finally divided into six groups of five teeth each.
Control group (CG): no treatment
G1: only diode laser irradiation
G2: only Er:YAG laser irradiation
G3: mechanical instrumentation plus NaOCl 5,25%
G4: same protocol of G3 plus diode laser
irradiation
G5: same protocol of G3 plus Er:YAG laser irradiation. A
quantitative evaluation using BioTimer method was
performed. Moreover, a qualitative analisys with BacLight®LIVE/DEAD viability probe and observation by epifluorescence microscopy was conducted.
Results: The treatment with the diode laser
greatly reduced the number and the size of bacterial aggregates that were not
immersed in extracellular matrix. The microscopy observations about the
bacterial cultures treated using the Er:YAG
laser revealed that bacterial aggregates were very rare. Conclusion: In every
case, laser irradiation improved the root canal disinfection. Er:YAG laser showed best results.