13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 91

TITLE: Evaluation of Healing of Third-Degree Burns in Diabetic and Non-diabetic Rats illuminated or not with a Polarized Light source.

AUTHORS: Marques APC, Oliveira PC, Reis Junior J, Castro I, Monteiro JSC, Pinheiro ALB.

Center of Biophotonics - School of Dentistry - Federal University of Bahia

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S239.

* doi:10.4317/medoral.17643739

http://dx.doi.org/10.4317/medoral.17643739

Abstract

Aim: The study evaluated, by light microscopy, the repair process on 3rd-degree burns on diabetic and non-diabetic rats, illuminated or not with a Polarized Light source. Background: Burns are severe injuries that result in the loss of fluid and destruction of tissue, infection, and shock that may result in death. Diabetes mellitus is a public health problem that, being uncontrolled, causes severe disturbance on the body metabolism, including on wound healing. Polarized light sources have been shown effective on improving healing on many situations. Materials and Methods: Ninety male Wistar rats were divided into 2 groups (n= 45). In one of the groups, diabetes mellitus was induced by streptozotocin. A 3rd degree burn, measuring 1.5X 1.5cm², was created in the dorsum of each animal. Phototherapy (λ400-2000nm, 20J/cm² or 40J/cm²) started immediately after burning and repeated daily until animal death (7, 14 and 21 days). Specimens were taken, processed and stained with H& E and Sirius Red and imunnomarked with CK AE1/AE3. Descriptive analysis was carried out by light microscopy. Results: Animals submitted to phototherapy showed an acceleration of the repair, being the dose of 20J/cm² the one that caused best results including higher deposition of collagen, quicker inflammatory reaction and improved revascularization. Conclusion: Our results suggest that the use of polarized light (20J/cm²) improved the healing of 3rd degree buns on both diabetic and non-diabetic animals.