13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 101

TITLE: Laser photo-stimulation of oral squamous cell carcinoma cell lines.

AUTHORS: Sperandio FF, Giudice FS, Aranha ACC, Corrêa L, Pinto Jr Ds, Scom S.

Department of Oral Pathology - School of Dentistry - University of Sao Paulo, Brazil

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S249.

* doi:10.4317/medoral.17643749

http://dx.doi.org/10.4317/medoral.17643749

Abstract

Laser photo-stimulation of cultured cells reveals different results, which may vary from proliferation to inhibition of cell growth. These results are influenced by the different nature of the cells and by the distinct parameters of the employed laser irradiation. This study aimed to evaluate the in vitro proliferation of neoplastic cells from two tongue squamous cell carcinoma cell lines after laser irradiation (SCC9 and SCC25 cell lines). Laser irradiation was performed with both red (660nm) and infrared light (780nm). The power was set in 40mW and the chosen energy densities were 2, 3 and 6 J/cm². A single irradiation was performed and the cell viability was assessed after 12, 24, 48 and 72 hours through the MTS assay. The results of this study showed that there were differences among the experimental groups regarding increased and often diminished cell viability of the laser groups. Statistical ana-lyses were performed through ANOVA and Tukey test revealing significant differences, especially between the experimental and the control groups. In that way, low-level laser irradiation as performed herein was able to modify the growing rates of oral squamous cell carcinoma cells in vitro.