13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 105

TITLE: The effect of low – level laser irradiation on malignant blood cells: an in vitro study.

AUTHORS: Hosseinkazemi H, Dastanpour S, Bitolahi J, Saber K.                    

Department of Oral Medicine, Tehran University of Medical Science

SOURCE:  Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S253.

* doi:10.4317/medoral.17643753

http://dx.doi.org/10.4317/medoral.17643753

Abstract

Objective: the aim of this study was to investigate of different doses (J/cm²) of 810nm laser irradiation on cell proliferation of acute myelogenous leukemia cell line (KG-1a), in vitro.

Background: laser phototherapy is used in the clinical treatment of chemotherapy – induced mucositis in the patients with leukemia, despite limited safety information. It has been suggested that laser light may have biostimulatory effect on tumor cells. This study contributes to systematically developing guidelines for the safe use of laser in chemotherapy – induced mucositis.

Methods: Human acute myelogenous leukemia cell line (KG-1a) were irradiated with a single, two and three exposures at the energies of 5, 10, 20 J/cm². Seven days after cell culture, cell proliferation was assessed.

Results: Although certain doses of laser increased KG-1a cell proliferation, multiple exposures had no effect on dose response relationships.

Conclusion: Before a definitive conclusion can be made regarding the safety of laser for chemotherapy – induced mucositis in leukemic patients, further in vivo research is required.