13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 108

TITLE: Effect of photobiomodulation in Tongue Mast Cells in Rats Wistars Irradiated with LED Red and IR.

AUTHORS: Monteiro JSC, Oliveira SCPS, Pires-Santos GM, Carvalho C, Santos JN, Pinheiro ALB.

Center of Biophotonics - School of Dentistry - Federal University of Bahia

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S256.

* doi:10.4317/medoral.17643756

http://dx.doi.org/10.4317/medoral.17643756

Abstract

Objective: This paper aimed to study the effect of LED Phototherapy – LED-Pht (λ630nm or λ850nm) on mast cells on the dorsum of the tongue of rodents using Toluidine blue staining. Background Data: Laser light is able to induce the release of mediators responsible for vasodilatation, such as the ones produced by mast cells. Animals and Methods: 60 Wistars rats were divided into 3: (I) Control, (II) IR-LED (λ850nm, 21.9J/cm², 73s, (III) Red- LED (λ630nm, 21.9J/cm², 73s). The specimens were taken after, 20, 45 and 60’ following irradiation. Total, degranulated and non-degranulated mast cells were counted and statistical analysis performed. Results: Both LED irradiated subjects showed significant difference when compared with control subjects on the total number, degranulated mast cells, and non-degranulated. When comparing the two groups of LED irradiated subjects, significant difference was seen on regards the total number of cells and degranulated mast cells, being IR-LED group the one with higher number of these cells. On the other hand, Red-LED irradiated subjects showed significant higher number of non-degranulated mast cells. Conclusion: Our results leads us to conclude that both Red and IR-LED light caused increased mast cell degranulation being IR-LED light the one that resulted on higher number of mast cells.