13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 126

TITLE: Investigation of biocompatibility of new laser light activated metalphthalocyanine phothosensitisers.

AUTHORS: Dimitrov SK, Dogandzhiyska VD, Gueorgieva TG, Mantareva VN, Angelov IP.

Medical University-Sofia, FDM, Department Operative Dentistry and Endodontics: Bulgarian Academy of Sciences, Institute of Organic Chemistry

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S274.

* doi:10.4317/medoral.17643774

http://dx.doi.org/10.4317/medoral.17643774

Abstract

Phothodynamic therapy is an appropriate method to dental application. It was successfully applied into endodontic treatment as subsidiary method. Good results are observed by treatment of periodontal diseases, tooth bleaching and caries prevention too.

Aim: The aim of this in vivo study was to investigate biocompatibility of new synthesed, laser light activated metalpthalocyanine phothosensitisers, implanted in rats connective tissue.

Methods: All substances /two type new phothosensitisers, Photosan and saline solution/ were put into polyethylene tubule and implanted in subcutaneous connective tissue of 72 rats Wistar, divided into 3 groups. Sections of hide over implanted tubules were irradiated 6 times in 12 hours with laser light /660nm/ for phothodynamic activation of substances. At 7,30,60 days of experiment 8 samples of each subgroup were prepared to hystomorphological investigation.

Results: After 7 days in subcutaneous connective tissue was determined moderate inflammatory reaction to phthalocyanines, commensurable with reaction to Photosan, but stronger than response to saline solution. Hystomorphological investigations at 30 and 60 days manifested gradually decrease of inflammatory reaction.

Conclusion: Hystomorphological investigations presented acceptable biocompatibility between rat connective tissue and examined phothosensitisers. The results are in correlation to our previous researches about cythotoxicity of phothosensitisers on biological cell cultures.